Single-cell dissection and multi-cohort validation identify a hypoxia-related prognostic signature with experimental verification in lung adenocarcinoma.

Among all immune cell subsets, only Macrophages_M1 showed a significant difference between groups (P = 0.028), whereas differences in infiltration levels of other immune cell types did not reach statistical significance.

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The risk score showed an increasing trend with higher T stage, and patients with T3–T4 disease exhibited significantly higher risk scores than those with T1–T2 disease ( Figures 5B,C ).

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