highly significantp < 0.0001
One-way ANOVA demonstrated a highly significant effect of incubation time on biofilm formation ( p < 0.0001, R 2 = 0.966).
One-way ANOVA demonstrated a highly significant effect of incubation time on biofilm formation ( p < 0.0001, R 2 = 0.966).
Dunnett’s multiple comparisons test further revealed that biofilm formation at 72 h was significantly higher than at 24 h (mean difference = – 0.279, p = 0.013), while the increase from 24 to 48 h did not reach statistical significance (mean difference = – 0.164, p = 0.108).