Modular integration of nanopore sequencing, alphafold modeling, and statistical design boosts 1-hydroxyphenazine yield in Pseudomonas aeruginosa strain KAEH25.

Factors with t -values above the standard significance limit but below the Bonferroni threshold—agitation speed, iron concentration, and phosphate buffer—were considered moderately significant, exerting weaker but detectable influences on the response (Fig. 7 ).

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