Development of Pentaplex Reverse Transcription Droplet Digital PCR Assay for Simultaneous Detection and Absolute Quantification of HIV-1, HIV-2, HCV, and HBV With Internal Control.

For HCV, although the LLoQ was determined at the highest tested concentration within the evaluated range, the progressively decreasing CV values observed across subsequent lower concentrations exhibited a clear trend, supporting the suitability of this concentration as the operational LLoQ. 3.5.

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