A comparative analysis of gut microbiome in dogs using short- and long-reads of 16S rRNA sequences reveals workflow-dependent biases.

Vet Res Commun · 2026 · PMC13379502 · PMID 42467133

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nominally significantp = 0.038actually significant

PERMANOVA analyses identified nominally significant effects of Age within the ONT dataset at the phylum (R2 = 0.204, p = 0.038), family (R2 = 0.154, p = 0.020), and genus (R2 = 0.139, p = 0.029) levels, whereas no nominally significant effects were detected in the Illumina dataset (Supplementary Table S2).

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