This difference was apparent not only in the different number of DEGs but also in the breadth of functional enrichment: glaucum showed 19 significantly enriched GO terms, whereas murinum showed 36 (Figure 2 ; Table S5 ), indicating that elevated temperature affected a wider range of functional categories in the allotetraploid.
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Diploid- and tetraploid wall barley exhibit alternative molecular responses but comparable reproductive performance in response to different ambient temperature regimes.
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