Barely Significant
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The length of the embryo culture in assisted reproductive technology does not have a major impact on newborn DNA methylation.

Biosci Rep · 2026 · PMC13402866 · PMID 42454427

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nominally significantP <0.05actually significant
No major changes in DNA methylation detected between newborns conceived by cleavage or blastocyst transfer Genome-wide comparison of DNA methylation levels between cleavage- or blastocyst-transfer newborns revealed nominally significant ( P <0.05) differential methylation at 31,180 and 25,540 CpG sites for cord blood and arteries, respectively (Supplementary Tables S1,S2).

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