approached significanceP =0.06
Thymidylate synthase stain area also correlated with cytoplasmic HIF-1 α and GLUT-1 stain area ( r s =0.73, P <0.0001; r s =0.49, P =0.02 respectively), whereas the correlation between TS stain intensity and nuclear HIF-1 α expression approached significance ( r s =0.39, P =0.06).