Development of a new protocol for 2-day generation of mature dendritic cells from human monocytes.

Cells harvested from cultures where the two-step differentiation and activation protocol was used showed significantly higher expression of CD83 and 3-fold increase in secretion of total IL-12 (this trend did not reach statistical significance due to the large differences in maximal levels of IL-12 detected) compared to cells cultured with GM-CSF and IL-4 plus proinflammatory mediators from the beginning of cell culture (Fig. 2 ).

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