A construct with fluorescent indicators for conditional expression of miRNA.

BMC Biotechnol · 2008 · PMC2569932 · PMID 18840295

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did not reach statistical significanceno p-value reported

Silencing Drosha led to the recovery of RFP expression in constructs with the pre-miRNA placed directly in their 3'-UTR (see pCAG-RFP in Figure 4 , p < 0.05 for both miR-E1-1 and miR-E-2-4) but lowered the RFP expression somewhat in constructs with the pre-miRNA embedded in an intron, although this did not reach statistical significance (see pCAG-RFPint in Figure 4 ).

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