Flow cytometric analysis and microsatellite genotyping reveal extensive DNA content variation in Trypanosoma cruzi populations and expose contrasts between natural and experimental hybrids.

cruzi parents and hybrids as a result of passage through a mammalian host, heat shock and nutritional stress, and although two of these changes were shown to be weakly significant, none were greater than the typical G1-0 peak CV (5–9%).

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The highly significant correlation between pairwise genetic distance and DNA content could therefore be seen as consistent with a model under which differences in DNA content are accrued gradually during independent clonal evolution of different strains.

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