A versatile 3D culture model facilitates monitoring of astrocytes undergoing reactive gliosis.

The perimeter of vimentin staining significantly increased in TGFβ1-treated astrocytes after 5 days in culture, compared to non-treated control cultures at the same time point ( Figure 6b ; p < 0.05), although increases were seen at days 10 and 15, these did not reach statistical significance.

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