A flanking gene problem leads to the discovery of a Gprc5b splice variant predominantly expressed in C57Bl/6J mouse brain and in maturing neurons.

Overall Gprc5b levels, determined with a primer set (A/a) that amplifies a region common to both Gprc5b_v1 and v2, showed a trend towards an increase on day 17 (1.8 fold; p = 0.14) after normalization to beta-actin levels ( Fig. 4A , 3 rd panel from top ).

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