The response to unfolded protein is involved in osmotolerance of Pichia pastoris.

Intracellular trehalose levels were in the same range as glycerol levels but showed a significant trend ( p ≤ 0.05) towards decreased concentrations at medium and high osmolarity growth conditions in the wt strain but slightly missed the threshold p -value in the 3H6 Fab expressing strain (Figure 1C and Additional file 1 ) The effect of osmolarity on the P. pastoris intracellular proteome As 2D-DIGE has already been successfully applied to monitor changes in the P. pastoris proteome upon growth at different temperatures [ 26 ], this method was also applied to track changes upon growth at elevated osmolarity.

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