Rational design of an orthogonal tryptophanyl nonsense suppressor tRNA.

Nucleic Acids Res · 2010 · PMC2965240 · PMID 20571084

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quite significantno p-value reported

When the C28-G42 base pair in AS3.1 is finally changed to G28-C42 to yield variant AS3.4, there is a quite significant gain in suppression activity in the presence of the ScWRS (Δ 100.8 U/mg) but little in the way of background activity (Δ 0.1 U/mg).

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