Daily timed sexual interaction induces moderate anticipatory activity in mice.

Although there is a notable trend toward increased AA at days 21, 28, and 35 ( Figure 2G ), it is not clear why the males in experiment #2 stopped showing increased AA for female access.

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Summing the amount of time occupied by high activity behaviors over hours ZT 5-9 (just prior to female access) across each measurement showed a trend toward increased activity at several time points, particularly in the upper quartile range, but the only significant increase in activity occurred on day 28 (Mann-Whitney Test, p<0.05) ( Figure 1E ).

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