Design of experiments approach to engineer cell-secreted matrices for directing osteogenic differentiation.

Ann Biomed Eng · 2011 · PMC3069311 · PMID 21120695

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hMSCs cultured on DM2 also showed a trend for increased IBSP expression over control groups from day 7 through 21. mRNA expression of osteocalcin ( BGLAP ), a common biochemical marker of bone formation, 27 exhibited a significant increase in hMSCs cultured on DM1-coated wells over all other groups at days 3, 7, 15, and 21 (Fig. 3 d).

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