However, we note a trend in the Sp5/Btd clade towards the accumulation of more proline, whereas in the Sp6-9 clade there is a clear trend towards accumulating serine and threonine in the N-terminal portion.
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A clustered set of three Sp-family genes is ancestral in the Metazoa: evidence from sequence analysis, protein domain structure, developmental expression patterns and chromosomal location.
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