approached significanceP = 0.06
No significant differences in circulating INSL3 concentration were observed for any day between gravid and non-gravid sheep ( Fig.1A ), although in the pregnant ewes the increase in INSL3 concentrations on day 12 compared to day 5 approached significance (P = 0.06), and values were close to being significantly different between gravid and non-gravid animals (P = 0.07) on day 17.