Quantification of endogenous IAA levels by gas chromatography–mass spectrometry showed a trend similar to that of the radiotracer studies ( Figure 3A ). phot1 mediated modest increases in IAA accumulation in the apical tissues of dark-acclimated seedlings that included the upper hypocotyl, petioles, and cotyledons ( Figure 3A ).
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phot1 inhibition of ABCB19 primes lateral auxin fluxes in the shoot apex required for phototropism.
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