Barely Significant
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Freezing of rat tibiae at -20°c does not affect the mechanical properties of intramedullary bone/implant-interface: brief report.

Open Orthop J · 2011 · PMC3134982 · PMID 21760868

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showed a trendno p-value reported
Group B (frozen/thawed) showed a trend towards a lower shear strength in the mechanical testing, which was not significant (average shear strength group B 0.45 ± 0.13 N/mm 2 vs. group A 0.63 ± 0.09 N/mm 2 ).

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