Novel gene expression responses in the ovine abomasal mucosa to infection with the gastric nematode Teladorsagia circumcincta.

However, the multiplex qRT-PCR analysis specific for complement factor 1 did not show the same pattern of expression detected in the arrays, with a lot of sheep-to-sheep variation, although there was a clear trend towards up-regulated gene expression in response to challenge in both groups in Expt. 1 (Figure 6 ).

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Immune animals demonstrated highly significant increases in levels of transcripts normally associated with cytotoxicity such as granulysin and granzymes A, B and H, as well as mucous-cell derived transcripts, predominantly calcium-activated chloride channel 1 ( CLCA1 ).

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