Validation and application of a PCR primer set to quantify fungal communities in the soil environment by real-time quantitative PCR.

For each soil sample, the relationship between the C T value and the logarithm of the amount of DNA template in the PCR was linear and highly significant (r 2 >0.99, Figure 2 , raw data are provided in Table S6 ).

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However, this underestimation should remain weakly significant because it is related mainly to basal fungal lineages which constitute a small proportion of the fungal taxa currently referenced in the fungal databases.

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