Importantly, plotting the difference in log-scores before and after co-transfection of Tra2β indicated a clear trend in splicing activation between pre-mRNAs: the splicing response of each of the pre-mRNAs to Tra2β was inversely correlated to 5′-splice site strength ( Figure 2 D).
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Molecular design of a splicing switch responsive to the RNA binding protein Tra2β.
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