The Y64F mutant doubled Rac1 interaction with βPIX ( Figure 6 , A and B), but the data on Tiam1 association revealed an increase in association with EGFP-Rac1-Y64F as compared with the wild type EGFP-Rac1 that did not reach statistical significance ( Figure 6 , C and D).
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Tyrosine phosphorylation of Rac1: a role in regulation of cell spreading.
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