qRT-PCR data for GTPase RhoA ( Figure 3J ) and phosphatidylinositol 4-kinase ( Figure 3K ) showed a trend similar to that of the array data ( Figure 2A ), where GTPase RhoA showed fewer transcripts at the 4 or 12 h time points, and phosphatidylinositol 4-kinase showed lower mRNA expression at the 12 or 24 h time points in E 2 -treated samples compared to controls ( Figure 3J and 3K ). qRT-PCR also showed a significant increase in phosphatidylinositol 4-kinase transcript numbers at 24 hours of transition in controls compared to E 2 -treated samples ( Figure 3K ).
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Influence of 17β-estradiol on gene expression of Paracoccidioides during mycelia-to-yeast transition.
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