Transcriptional profiling of innate and adaptive human immune responses to mycobacteria in the tuberculin skin test.

Functional annotation clustering analysis of the gene list that generated the top 10% of the total variance, identified from the PCA (factor) loadings in PC1, showed highly significant enrichment for gene ontology clusters involving immune responses ( Fig. 1C ).

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In each case, gene expression was greater in 48-h samples from TST + subjects compared to TST − subjects, although this did not reach statistical significance for IL12B.

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