The number of scratches appeared to be higher in the higher dose group (50 μg) compared to the vehicle group, but the numbers did not reach statistical significance (vehicle = 110.7 ± 11.9, 50 μg of drug = 136.6 ± 8.2; unpaired t -test, p = 0.08, Figure 5A ).
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Genetic enhancement of behavioral itch responses in mice lacking phosphoinositide 3-kinase-γ (PI3Kγ).
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Interestingly, the formalin graph shows a slight trend towards an increased pain response in PI3Kγ KO mice during the phase 3, but the mean difference was not statistically significant (WT = 165.9 ± 35.6 sec, KO = 262.3 ± 51.9 sec; unpaired t -test, p > 0.05 , Figure 2A-B ).