Small cationic DDA:TDB liposomes as protein vaccine adjuvants obviate the need for TLR agonists in inducing cellular and humoral responses.

Median responses after DRV(OVA) vaccination were higher for both CD8 and CD4 splenocytes as compared with MLV(OVA) liposomes, but did not reach statistical significance ( Figure 2A, 2B and 2C ).

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The DSPC/TDB liposomes had no significant effect on the cellular immunogenicity induced by MVA in the spleen, although a decreasing trend was observed with MLVs for both CD4 and CD8 epitopes when compared to MVA-only vaccination ( Figure 4A, 4B, 4C ).

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