Tumor necrosis factor alpha mediates GABA(A) receptor trafficking to the plasma membrane of spinal cord neurons in vivo.

Quantitative Western Blot Detects a Nonsignificant Trend of Increased GABA A R in Total Membrane Fractions Quantitative western blotting was used to generate normalized GABA A R intensity ratios (GABA A R : NCad) for the membrane-enriched fraction of spinal cord homogenate ( Figure 3(a) ).

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Whole membrane fractionation and quantitative western blotting showed a trend towards increased membrane GABA A R protein levels within 60 min of TNF α nanoinjection delivery, but these results did not reach significance.

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