A novel HPLC method for the concurrent analysis and quantitation of seven water-soluble vitamins in biological fluids (plasma and urine): a validation study and application.

Additionally, concentrations of vitamins were stable in processed (deproteinised) plasma samples and water-based controls for 24 hours when stored at +4°C prior to analysis [difference between peak area of vitamins in standard samples and peak area of vitamins in processed samples did not reach statistical significance when stored at +4°C for 24 hours, Student's t -test not significant, P > 0.05].

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