The crest phenotype in chicken is associated with ectopic expression of HOXC8 in cranial skin.

We found that HOXC8-ssr , HOXC8-3end and HOXC11 all showed highly significant significant linkage to Crest ( P = 5.5×10 −33 ) with no recombination event detected ( Fig. 2 , Table S3 and S4 ).

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Expression analysis by quantitative PCR (q-PCR) analysis using different skin tissues from embryonic stage E13 to E21 showed a trend of increased expression as the embryo developed ( Fig. 3B&C ).

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