Similar experiments using siRNA showed that whereas RGDfV tended to increase ceramides C 16 and C 18 in cells with non-silencing control siRNA (p-values approaching significance), in cells with knocked down c-Abl there was no difference in ceramides C 16 and C 18 in presence or absence of RGDfV ( Fig. 5E ). siRNAs to ASM (ASM1, ASM2) however, were not able to block RGDfV-induced phosphorylation of c-Abl at Y412, nor did they change c-Abl protein expression ( Fig. 5F–G ).
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c-Abl is an upstream regulator of acid sphingomyelinase in apoptosis induced by inhibition of integrins αvβ3 and αvβ5.
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