Similarly, ALP and OCN were upregulated (over static culture) by 143% ( P = 0.04) and 1072% ( P = 0.07), respectively, when cocultures were exposed to perfusion flow ( Table 4 ), although OCN only approached significance.
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Perfusion flow enhances osteogenic gene expression and the infiltration of osteoblasts and endothelial cells into three-dimensional calcium phosphate scaffolds.
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