Expression of the human glucokinase gene: important roles of the 5' flanking and intron 1 sequences.

A small increase with fetal calf serum was seen in the pGK-1049Intr1Luc intron-containing construct, but it did not reach statistical significance and the intron containing construct with the longest 5′ flanking region, pGK-3815Intr1Luc had minimal activity under all treatments ( Fig. 2B ).

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