only marginally significantp = 0.047
Assaying 25 μM NAD + with 80, 120, 160 and 200 μg/ml ADH, we found ADH concentration (mg/ml) and V mean to have a weak and only marginally significant relationship ( V mean = 379+0.558[ADH], p = 0.047, n = 8). 65°C Incubation for 30 Minutes is not Sufficient to Fully Degrade NAD + Thirty minutes of 65°C incubation was shown to degrade NAD + while keeping NADH intact [24] , as NAD + has a higher temperature dependent degradation rate than NADH at pH around 7.0 [24] .