We measured free amino acid levels from heads of Rheb overexpressing flies (using the neuronal driver elav-Gal4 ) and found significantly higher levels of several amino acids and their metabolites, however, although tyrosine levels showed a trend towards increase in Rheb overexpressing samples, the change did not reach statistical signficance ( Fig. 3D ).
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The TSC1/2 complex controls Drosophila pigmentation through TORC1-dependent regulation of catecholamine biosynthesis.
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