showed a trendP = 0.07
Increased number of involved lymph nodes (more than 3) correlated with elevated expression of TWIST1 protein in LNM (P = 0.02) and showed a trend towards increased expression of TWIST1 mRNA in LNM (P = 0.07) and SNAIL protein in LNM (P = 0.07) (in Additional file 5 : Table S2).