However, when using only 100 pairings, LTD is more sensitive to timing requirements: LTD was induced best when PF stimulation preceded CF activity by 250 ms, a depression that did not reach statistical significance resulted from PF+CF co-activation with 125 ms or 0 ms intervals, and no change was observed when CF stimulation preceded PF activity by 250 ms (Chen and Thompson, 1995 ).
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