An efficient rRNA removal method for RNA sequencing in GC-rich bacteria.

Microb Inform Exp · 2013 · PMC3563489 · PMID 23294941

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quite significantno p-value reported

While samples only treated with the MICROBExpress kit showed very high correlation with total RNA (r = 0.979), treatment with the Ovation kit reduced the correlation with the control (r = 0.714), suggesting that the selective amplification methodology results in the introduction of a quite significant bias in mRNA relative abundance.

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