slightly significantP = 0.047
When the second year was included, the results were slightly significant as a whole (acrosome integrity: P = 0.047; morphology: P = 0.050), but not between any treatment from the first collection year and the 7% glycerol with centrifugation treatment of the second year, except the 5% glycerol with quail egg yolk treatment for morphology (see Supplementary material, table S3 ).