Consistent with the previous unsupervised analysis, a histogram of P -values from the supervised analysis showed a clear trend towards small significant P -values (Figure 2A ).
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Identification and functional validation of HPV-mediated hypermethylation in head and neck squamous cell carcinoma.
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The skew towards hypermethylation (seen in the described experiment on FFPE HNSCCs) was confirmed to be highly significant in E6 and E6 & E7 clones against the background probability of hypermethylation (there was widespread hypermethylation in E6 and E6 & E7 clones) (Figure 5A , Monte Carlo, P = 0.007).