Ferrous iron is a significant component of bioavailable iron in cystic fibrosis airways.

Here, we found a highly significant negative correlation between absolute Fe(II) concentrations and disease status (ρ = −0.56, P = 0.004) ( Fig. 1B ), though a similar relationship was not found for Fe(III) (ρ = −0.28, P = 0.179) ( Fig. 1C ).

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Treating each sputum sample independently, we found a strong trend between PCA abundance and Fe(II) % (ρ = 0.185, P = 0.069), yet no correlation between PYO abundance and Fe(II) % (ρ = 0.042, P = 0.67) ( Fig. 2B and C ).

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