Barely Significant
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Multiplex qPCR for detection and absolute quantification of malaria.

PLoS One · 2013 · PMC3756973 · PMID 24009663

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showed a trendno p-value reported
In general, data showed a trend where as the reaction volume increased, qPCR assays became slightly less efficient for all the assays with a plateau being reached at reaction master mix of 8 μl.

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