Generalized bacterial genome editing using mobile group II introns and Cre-lox.

Mol Syst Biol · 2013 · PMC3792343 · PMID 24002656

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Insertion of the entire operon into the lacZ gene was facile (as judged by PCR across an insertion junction) and also showed a trend of increased insertion efficiency as a function of incubation time; after 3 days of incubation, 25/25 screened colonies tested positive for the insertion.

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