An RNAi-based approach to down-regulate a gene family in vivo.

A small reduction in the number of B220-positive cells was observed in the presence of the recombinase, but this phenotype did not reach statistical significance, therefore it might represent a slight tendency, but does not proof phenotypic changes due to the expression of the Cre recombinase.

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Statistical analysis shows that the described differences in the expression of Gfp are highly significant, and also indicate that the number of B220-positive cells is reduced from 53.8% in wild-type animals to 40.4% in vav Cre ::Rosa26 RNAi/+ and 38.6% in vav Cre ::Rosa26 RNAi/RNAi mice, indicating a potential but weak influence of the transgene on B cell development ( Figure 3B ).

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