approaching significanceP = 0.08
Further, when the firing rate of MHb neurones from these two gentoypes were compared in two 4 h time bins corresponding to projected ZT 0–4 and 8–12, two-way ANOVA indicated a significant effect of genotype ( F (1,63) = 5.8; P < 0.05) and time × genotype interaction ( F (1,63) = 5.1; P < 0.05), with the effect of time approaching significance ( F (1,63) = 3.2; P = 0.08).