only marginally significantP = 0.01
This pattern of increased autosomal expression was strongest in the unfiltered data set (Wilcoxon tests; female P = 1.52 × 10 −6 ; male P = 5.78 × 10 −5 ), but average expression differences and strength of significance reduced rapidly with a small increase in filtering threshold: Although differences were significant when filtering at 2 FPKM (female P = 6.26 × 10 −6 ; male P = 2.02 × 10 −4 ), they were only marginally significant at 3 FPKM (female P = 0.01; male P = 0.05) and nonsignificant when filtering at 4 FPKM (female P = 0.12; male P = 0.96; fig. 2 b ; table 1 ; supplementary figs.