Microinjection of Cy3-p110αEE WT /p85α or Cy3-p110αEE H1047R /p85α resulted in a small increase in the proportion of cells with protrusions, which was also further increased in stimulated cells; however, the difference between p110αEE WT /p85α and Cy3-p110αEE H1047R /p85α was marginal and probably not significant.
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Assessing the subcellular distribution of oncogenic phosphoinositide 3-kinase using microinjection into live cells.
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