For genes where Bartlett test for equal variance found the difference between variances to be highly significant (P < 0.001) between the three folliculogenesis phases, the data was transformed using the formula Y = Log(Y *1e12), where multiplying by a large factor was meant to obtain values higher than 1, as the log of a proportion between 0 and 1 would give a negative value, and simply multiplying by -1 would not respect the original relative expressions.
G dataset, with cell death, cell survival, colony survival of cells and cell viability of tumor cell lines having a decreasing trend and Apoptosis showing an increasing trend, albeit none of them were statistically significant (Figure 4 B).
IPA calculates a z-score for each functional category where a positive z-score predicts that the biological process is trending towards an increase, and a negative z-score corresponds to a decreasing trend.
There is a clear trend towards the down-regulation of later phases of the cell cycle such as S-, G2- and M-phase, as well as cytokinesis, while the resting G1 phase is showing an increasing trend.